| Watershed | Sampling Method | Sample Date | Collector(s) | Determiner |
|---|---|---|---|---|
| Big Creek | by hand | 1996-06-26 | I & M Aquatics Crew | Pennington & Associates |
| Bradley Fork | by hand | 1995-06-14 | GSalansky & HSoehn | Pennington & Associates |
| Bunches Creek | by hand | 1994-09-01 | GSalansky & JLButcher | Pennington & Associates |
| Cataloochee Creek | by hand | 1994-07-22 | GSalansky & JLButcher | Pennington & Associates |
| Cataloochee Creek | by hand | 1995-07-18 | GSalansky & HSoehn | Pennington & Associates |
| Cataloochee Creek | by hand | 1995-09-13 | GSalansky & HSoehn | Pennington & Associates |
| Cosby Creek | by hand | 1995-06-12 | GSalansky & HSoehn | Pennington & Associates |
| East Prong Little River | by hand | 1995-09-18 | GSalansky & HSoehn | Pennington & Associates |
| East Prong Little River | by hand | 1996-09-19 | I & M Aquatics Crew | Pennington & Associates |
| East Prong Little River | by hand | 2001-07-01 | I & M Aquatics Crew | American Aquatics |
| Middle Prong Little River | by hand | 1995-06-20 | GSalansky & HSoehn | Pennington & Associates |
| Straight Fork | by hand | 1994-08-24 | GSalansky & JLButcher | Pennington & Associates |
| Uncertain | by hand | 1991-05-25 | DAEtnier | JWojtowicz |
| West Prong Little Pigeon | by hand | 1995-07-05 | GSalansky & HSoehn | Pennington & Associates |